Figure 3.

Evaluation of CcCKS-transgenics against different abiotic stresses. Seed germination rate (A) of WT, VT, and transgenic plants under normal, 150 mM mannitol and 125 mM NaCl stress. Two-week-old seedlings of WT, VT, and transgenics were grown on 150 mM mannitol and 125 mM NaCl for 7 days and were later allowed to recover on MS plates. Data on survival rate (B), total biomass (C) and root length (D) were recorded after 10 days of recovery. In each treatment, 20 seedlings of WT, VT, and two transgenic lines were used. Chlorophyll content (E) was determined from the leaf disks of control and transgenics after 72 h of incubation in water, 150 mM mannitol and 125 mM NaCl solutions independently at room temperature (20 ± 1°C). Bar represents mean and error bars represents SE from three independent experiments. Asterisks indicate significant differences in comparison with the WT at P < 0.05. WS, without stress; CS1, CS2, and CS3: three different transgenic lines; WT, wild type; VT, vector transformed plants.