Supplementary Table 1.
Fluorochromes Used for Identification of Immune Cells by Flow Cytometry
| Marker | Fluorochromes | Company | Reference no. |
|---|---|---|---|
| CD4 | V450 | BD Biosciencesa | 560468 |
| CD8a | APC-Cy7 | BD Biosciencesa | 561967 |
| CD11b | PE | BD Biosciencesa | 557397 |
| CD11c | APC | BD Biosciencesa | 550261 |
| CD45R/B220 | FITC | BD Biosciencesa | 553087 |
| Ly-6G and Ly6C (Gr-1) | PerCP-Cy5.5 | BD Biosciencesa | 552093 |
| Immune cell | Fluorochromes |
|---|---|
| Th CD4+ | V450+/APC-Cy7- |
| CTL CD8+ | V450-/APC-Cy7+ |
| CD45R/B220+ B cells | CD8a-/CD45R/B220+ |
| CD11b+Ly6C/Gnet-low macrophages | CD11b+/Ly-6G and Ly6C (Gr-1) |
| CD11b+Ly6C/G+high neutrophils | CD11b+/Ly-6G and Ly6C (Gr-1) |
| CD11c+ DCs | CD4+/CD11c+, CD8a+/CD11c+-, CD4-/ CD8a-/CD11c+ |
a
BD Biosciences, San Diego, CA.