Fig. 2.

Fluorescence optical property correction phantoms and verification with in-vivo and ex-vivo comparison. (a) Fluorescence SVD amplitude for phantom experiments with varying optical properties and the same BPD concentration ($0.25\mathrm{mg}/\mathrm{kg}$). The best-fit (shown as dashed lines) uses the form $a/\mathrm{CF}$, where CF is Eq. (2) and $a=7.645$. (Note, $\mathrm{CF}=1$ is normalized for ${\mu }_{\mathrm{a}}=0.69$, ${\mu }_{\mathrm{s}}^{\prime }=11{\mathrm{cm}}^{-1}$.) (b) BPD concentration (in $\mu \mathrm{M}$) versus the corrected SVD (${\mathrm{SVD}}_{\mathrm{corr}}$). The line of best-fit $[\mathrm{BPD}]=(0.0301\pm 0.0009)\times {\mathrm{SVD}}_{\mathrm{corr}}$ with $R^2=0.9986$ is used to convert ${\mathrm{SVD}}_{\mathrm{corr}}$ to [BPD] in $\mu \mathrm{M}$. (c) The measured in-vivo photosensitizer concentration using the multifiber contact probe obtained fluorescence spectra versus ex-vivo measured BPD concentration. The line of best-fit is of the form $y=0.9821x$ with $R^2=0.9772$. The dashed line in (c) represents the line for $y=x$.