Figure 3.

Ligation of CD180 inhibits IFN-α-induced expression of ISGs. (a–c) Isolated murine splenic B220⁺ B cells were stimulated with anti-CD180 antibody (0.2 μg mL⁻¹) or mouse IFN-α (1000 U mL⁻¹). qPCR analysis of the expression of IFIT1, MX1, and TLR7 at 4 h (a) and 8 h (b). Western blot analysis of the protein levels of TLR7 and STAT-1 at 24 h (c). The data shown represent the mean values of three independent experiments, and the error bars represent the SEM. *p < 0.05, **p < 0.01, ***p < 0.001, as determined by ANOVA; ns denotes p > 0.05.