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Ligation of CD180 inhibits the IFN-α-induced phosphorylation of STAT-2. (a–c) Isolated murine splenic B220+ B cells were stimulated with anti-CD180 (0.2 μg mL−1) or mouse IFN-α (1000 U mL−1) for 10 min, 20 min, and 40 min. Western blot analysis of the tyrosine phosphorylation of JAK1 (Y-1022) (a), tyrosine phosphorylation of STAT-1 (Y-701) (b), serine phosphorylation of STAT-1 (S-727), (c) and tyrosine phosphorylation STAT-2 (Y-690) (d).
