NKp80 and NKG2D expression levels were upregulated on second trimester dNK cells. (a) Representative histograms of multiple dNK cell activation marker expression from the 8th (first trimester; solid line) and 14th (second trimester; dotted line) week deciduae. Viable CD45+CD56+CD16− dNK cells were gated. A fluorescence minus one (FMO) control is shown in gray. (b) NKp46, NKp44, NKp30, 2B4, NKG2D, and NKp80 expression on dNK cells from 6- to 20-week decidual samples (n = 65). (c) The average percentage of these activation markers between the first and second trimester dNK cells. n = 34 (first trimester; 9 ± 1.9 week) or n = 31 (second trimester; 16 ± 1.5 week). *p < 0.05.