Immunological staining of dNK cells and trophoblast during the first and second pregnancy trimesters. (a) Representative immunohistochemistry of CD45, CD56, HLA-G, and CK7 to visualize the localization of CD45+ decidual leukocytes, CD56+ dNK and HLA-G+ EVT, CK7+ trophoblasts. (b) Co-staining images of dNK (CD56+; green) and trophoblast (CK7+; red) cells. Scale bar = 100 μm. n = 22 (first trimester; 9 ± 2.0 week) or n = 14 (second trimester; 15 ± 1.7 week).