Figure 6.

Trophoblast engagement drives dNK survival and proliferation. Decidual leukocytes were stained with CFSE and cultured for 6 days in complete RPMI 1640 medium with 10% FBS in the presence of (i) trophoblastic HTR-8 cells; (ii) HTR-8 CM; (iii) cytokine mixture of rhIL-2 (2 ng mL⁻¹) and rhIL-15 (10 ng mL^{−1). No additional stimulatory cytokines were added to the HTR-8 or CM groups. Viable CD45+CD56+ dNK cells were gated, and their proliferation was assessed. n = 10 with average an gestational age of 11 ± 2.7 week.}