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. 2015 Aug 17;14(2):203–213. doi: 10.1038/cmi.2015.66

Figure 7.

Figure 7

Trophoblast interactions can change the dNK phenotype and function. (a) first and second trimester decidual leukocytes were cultured alone in RPMI 1640/10% FBS (control; red lines), with HTR-8 trophoblast cells (black lines) or HTR-8 CM (blue lines) for 16 hours, and then re-stimulated with cell stimulation cocktail (PMA/ionomycin/brefeldin A) and CD107a antibody for 4 hours before being processed with intracellular cytokine staining. Viable CD56+CD16− dNK cells were gated to assess their NKp80, NKG2D, IFN-γ, and CD107a expression levels. An FMO control was included (gray line). (b) The summarized results of the dNK cell NKp80, NKG2D, IFN-γ, and CD107a expression levels during the first and second pregnancy trimesters. n = 15 from the first trimester (9 ± 1.7 week); n = 11 from the second trimester (16 ± 1.8 week). *p < 0.05.