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. 2015 Jul 20;14(2):214–222. doi: 10.1038/cmi.2015.67

Figure 3.

Figure 3

Smo agonist and antagonist do not affect FLSs apoptosis. FLSs were cultured and serum-starved for 18 hours before incubation with GDC-0449 (5 μM) or purmorphamine (5 μM) for 48 hours. Cell apoptosis was determined by Annexin V and PI staining. Populations in the lower-right quadrant of the flow cytometric graph represented early apoptotic cells (a). The percentage of apoptotic cells (b) and live cells (c) was quantified. Data are represented as means ± S.D. of three independent experiments. NS: not significant.