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. 2015 Jul 20;14(2):214–222. doi: 10.1038/cmi.2015.67

Figure 5.

Figure 5

Knockdown of Smo by siRNA affects the mRNA and protein expression of cell cycle-related genes. The level of mRNA expression of cyclin D1 (a), cyclin E1 (b), and p21/CIP1 (c) were evaluated using real time-PCR. Relative quantification of gene expression was performed by the 2−ΔΔCt method. The level of protein expression of cyclin D1 (d) was estimated using western blot analysis. Expression of cyclin D1 protein was quantified using GAPDH as an internal control (e). Data are represented as means ± S.D. of three independent experiments. *P < 0.05 versus NC-siRNA group. p21/CIP1: p21/cyclin-dependent kinase-interacting protein 1.