Skip to main content
. 2015 Sep 21;14(2):223–234. doi: 10.1038/cmi.2015.84

Figure 1.

Figure 1

DAMP induction of BAM-SiPc-PDT in vitro. (a) Cytotoxicity. Different concentrations of BAM-SiPc were incubated with CT26 cells. The cytotoxic effects of BAM-SiPc against the CT26 cells with or without light illumination are shown as the means with S.E.M. of three independent experiments. (b) DAMP expression (confocal microscopy). PDT was performed with 10 nM BAM-SiPc, and the cells were allowed to stand for 4 h. CT26 cells were stained with Hoechst 33342 (blue), an antibody against cadherin (red) and antibodies against CRT, HSP70, or HSP90 (green). Arrows indicate the locations of DAMPs. Bar = 10 μm. (c) DAMP expression (flow cytometry). At 4-h post-PDT, cells were stained with propidium iodide and antibodies against various DAMPs. The population undergoing immunogenic cell death (propidium iodide−ve/DAMPs+ve) is shown as the mean with S.D. of three independent experiments. *p < 0.05; **p < 0.005.