Table-2.
Biochemical characteristics of suspected isolated from naturally infected hybrid tilapia (Oreochromis spp.) using Vitek 2 with 99% probability of S. agalactiae.
| Well | Biochemical test | Result |
|---|---|---|
| 2 | D-amygdalin (AMY) | − |
| 4 | Phosphatidylinositol phospholipase C (PIPLC) | − |
| 5 | D-xylose (dXYL) | − |
| 8 | Arginine dihydrolase 1 (ADH1) | + |
| 9 | Beta-galactosidase (BGAL) | − |
| 11 | Alpha-glucosidase (AGLU) | − |
| 13 | Ala-Phe-Pro arylamidase (APPA) | − |
| 14 | Cyclodextrin (CDEX) | − |
| 15 | L-aspartate arylamidase (AspA) | − |
| 16 | Beta galactopyranosidase (BGAR) | − |
| 17 | Alha-mannosidase (AMAN) | − |
| 19 | Phosphatase (PHOS) | + |
| 20 | Leucine arylamidase (LeuA) | + |
| 23 | L-proline arylamidase (ProA) | − |
| 24 | Beta glucuronidase (BGURr) | − |
| 25 | Alpha-galactosidase (AGAL) | − |
| 26 | L-pyrrolidonyl-arylamidase (PyrA) | − |
| 27 | Betaglucuronidase (BGUR) | − |
| 28 | Alanine arylamidase (AlaA) | − |
| 29 | Tyrosine arylamidase (TyrA) | − |
| 30 | D-sorbitol (dSOR) | − |
| 31 | Urease (URE) | − |
| 32 | Polymixin b resistance (POLYB) | + |
| 37 | D-galactose (dGAL) | + |
| 38 | D-ribose (dRIB) | + |
| 39 | L-lactate alkalinization (ILATk) | − |
| 42 | L-actose (LAC) | − |
| 44 | N-acetyl-d-glucosamine (NAG) | + |
| 45 | D-maltose (dMAL) | + |
| 46 | Bacitracin resistance (BACI) | + |
| 47 | Novobiocin resistance (NOVO) | + |
| 50 | Growth in 6.5% NaCl (NC 6.5) | + |
| 52 | D-mannitol (dMAN) | − |
| 53 | D-mannose (dMNE) | + |
| 54 | Methyl-B-D-glucopyranoside (MBdG) | + |
| 56 | Pullulan (PUL) | − |
| 57 | D-raffinose (dRAF) | − |
| 58 | O/129 resistance (comp. vibrio) (O129R) | − |
| 59 | Salicin (SAL) | − |
| 60 | Saccharose/sucrose (SAC) | + |
| 62 | D-trehalose (dTRE) | + |
| 63 | Arginine dihydrolase 2 (ADH2s) | + |
| 64 | Optochin resistance (OPTO) | + |
S. agalactiae=Streptococcus agalactiae