Figure 4.

Hepatocyte-activated nucleoprotein-specific CD8⁺T cells in uninfected transthyretin-nucleoprotein (TTR-NP) mice do not express proapoptotic Bim protein. (A) 2 × 10⁶ carboxyfluorescein diacetate succinimidyl ester (CFSE)-labeled naive CD8⁺ NP_396–404-specific T cells isolated from TNP4 mice were transferred IV into uninfected TTR-NP and B6 mice. Two days after the adoptive transfer, the proliferation of CFSE-labeled cells was assessed. (B) Expression of activation markers CD62L, CD25, and CD69 was assessed on NP_396–404-specific T cells 2 days after adoptive transfer in TTR-NP or B6 mice. (C) Isolated CFSE-labeled NP_396–404 and GP_33–41-specific CD8⁺ T cells from TNP4 and P14 transgenic mice, respectively, were adoptively transferred into TTR-NP and B6 mice and analyzed for Bim expression by flow cytometry 2 days after transfer. (Representative data of two independent experiments are shown; n = 4 per group.)