Figure 6.

Hepatocyte-expressed NP is efficiently presented and leads to CD8⁺T-cell activation. (A) GP_33–41- and NP_396–404-specific CD8⁺ T cells isolated from P14 and TNP4 TCR transgenic mice and labeled with CFSE or efluor450, respectively, were adoptively transferred (1.5 × 10⁶ cells) into transthyretin-nucleoprotein (TTR-NP) and B6 mice before low-dose infection with lymphocytic choriomeningitis virus (LCMV)-WE. Proliferation was assessed by flow cytometry 5 days after infection. (B) GP_33–41- and NP_396–404-specific CD8⁺ T cells were labeled as described and were adoptively transferred into recombination-activating gene (RAG)-deficient or RAG-deficient TTR-NP mice to monitor the proliferation in absence of infection. Proliferation was assessed 7 days later by flow cytometry. The GP_33–41 (CFSE) and NP_396–404 specific (efluor450) CD8⁺ T cells before transfer (dashed lines) are shown as controls. (Representative data of two independent experiments are shown; n = 4 per group.) **P < .01.