Figure 7.

Interleukin-10 (IL-10)-secreting CD4⁺FoxP3⁺regulatory T cells expand after lymphocytic choriomeningitis virus (LCMV) infection of transthyretin-nucleoprotein (TTR-NP) mice. (A) T-cell proliferation of adoptively transferred (1 × 10⁸ T cells from B6 mice) into recombination-activating gene (RAG) and RAG-NP mice monitored by Ki-67 expression 7 days after transfer. (B) Number of CD4⁺CD25⁺FoxP3⁺ regulatory T cells in spleen (left panel) and liver (right panel) before and at 8 days after lymphocytic choriomeningitis virus (LCMV)-WE infection. (C) Proportions of CD4⁺CD25⁺FoxP3⁺ regulatory T cells in the spleen (left panel) and liver (right panel) at 55 days after infection. (D) Expression levels of Helios on CD4⁺CD25⁺FoxP3⁺ regulatory T cells in infected TTR-NP (solid line) and B6 mice (dotted line) at 55 days after LCMV-WE infection. (E) Serum levels of IL-10 in infected TTR-NP and B6 mice 8 to 147 days after LCMV-WE infection. (F) Secretion of IL-10 by FoxP3⁺-expressing cells among total splenocytes from TTR-NP mice at 55 days after LCMV-WE infection (left), and the proportion of IL-10 secreted by CD4⁺FoxP3⁺ regulatory T cells in infected TTR-NP mice (right). (Representative data of two to three independent experiments are shown; n = 4–8 per group.) *P < .05, **P < .01, ***P < .001.