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. 2017 Feb 10;7:42279. doi: 10.1038/srep42279

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The cellular localization of Gβ after stimulation by gradients of fMLF (A) or niacin (B) or both (A-bottom raw) on RAW264.7 cells using zigmond chamber assays in revealed by staining with rabbit anti-Gβ antibodies followed by an Alexa 568-conjugated anti-rabbit igG secondary antibody. F-actin was stained with FITC-labeled phalloidin (bar: 10 μM). (C) Cell chemotaxis was inhibited by the Gβγ inhibitor gallein. (D) Cell chemotaxis was not inhibited by niacin in HCA2/FPR1/Gβ1 and HCA2/FPR1/Gβ4 transfected HEK293T cells.