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. 2017 Feb 10;7:42279. doi: 10.1038/srep42279

Figure 6. Niacin induces internalization and heterologous desensitization of FPR1.

Figure 6

(A) HEK293 cells co-expressing FPR1-EGFP and flag-HCA2 were loaded with the calcium probe Fura-2/AM, followed by the first or second stimulation (15 min later) of 5 μm fMLF with or without pretreated with 300 μm niacin, then calcium mobilization was assayed by monitoring the change in Fura-2/AM fluorescence. All data shown are representative of at least four independent experiments. (B) HEK293 cells co-expressing FPR1-EGFP and flag-HCA2 were stimulated with 5 μm fMLF with or without 300 μm niacin for 15 min or 30 min, then internalization of FPR1-EGFP was observed by confocal. (C) ELISA measurement of FPR1 remaining on the cell surface after treatment of cells by 5 μm fMLF with or without 300 μm niacin for 15 min or 30 min. Error bars, S.E. for four replicates. Data were analyzed using Student’s t test (**p < 0.01;***p < 0.001).