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. 2017 Feb 10;7:42427. doi: 10.1038/srep42427

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RT-qPCR was carried out for each of the 13 reference genes using the same RNA samples from control groups (PBS 1%, DNBS + PBS 1%, Ethanol 30%) and DNBS-induced colitis group (DNBS + Ethanol 30%). CT values were used to calculate the comprehensive gene stability ranking.