Figure 6. Alterations in the expression levels of the potential reference genes in colon of control groups (PBS 1%, DNBS + PBS 1%, Ethanol 30%) and DNBS-induced colitis group (DNBS + Ethanol 30%).

The expression levels of these genes in colonic samples were normalized to the Ct values of ERCC-00113, an external control RNA. The graphs show relative mRNA expression values that calculated using the ΔΔCt method. One Way ANOVA analysis followed by the multiple comparisons post hoc analysis were used to compare the groups with significance level 0.05. Data are shown as mean ± S.D. (n = 6/group).