siRNA primers |
MT1-MMP siRNA (NM_004995.2) |
5′-CCUACGAGAGGAAGGAUGGCAAAUU-3′ |
Control siRNA |
5′-UUCCUCUCCACGCGCAGUACAUUUA-3′ |
Primers for qRT-PCR |
E-cadherin |
5′-TTGACGCCGAGAGCTACA-3′ |
5′-GACCGGTGCAATCTTCAAA-3′ |
N-cadherin |
5′-ACCAGGTTTGGAATGGGACAG-3′ |
5′-ATGTTGGGTGAAGGGGTGCTTG-3′ |
Vimentin |
5′-TGAAGGAGGAAATGGCTCGTC-3′ |
5′-GTTTGGAAGAGGCAGAGAAATCC-3′ |
Snail |
5′-ATCGGAAGCCTAACTACAGCGAGC-3′ |
5′-CAGAGTCCCAGATGAGCATTGG-3′ |
Fibronectin |
5′-GATAAATCAACAGTGGGAGC-3′ |
5′-CCCAGATCATGGAGTCTTTA-3′ |
MT1-MMP |
5′-CGCTACGCCATCCAGGGTCTCAAA-3′ |
5′-CGGTCATCATCGGGCAGCACAAAA-3′ |
GAPDH |
5′-CATGAGAAGTATGACAACAGCCT-3′ |
5′-AGTCCTTCCACGATACCAAAGT-3′ |