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. 2017 Feb 10;7:42065. doi: 10.1038/srep42065

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Copyright © 2017, The Author(s)

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Panel (A) UV elution profile of CyaA from the size exclusion chromatography column Bio SEC-3 equilibrated in buffer A complemented with 4 mM CaCl₂ in-line with the SAXS measuring cell. Panel (B) distance distribution function P(r) derived from hCyaAm scattering pattern scaled to I(0). The dashed green line corresponds to the P(r) curve obtained by injecting the sample into the size exclusion chromatography column equilibrated with buffer A complemented with 2 mM EDTA. Panel (C) dimensionless Kratky plot of hCyaAm scattering pattern (red line) and PolX scattering pattern (black line, PolX is a compact, fully structured, globular protein⁸⁰). Panel (D) Two views of the most typical DAMMIN model of CyaA. Top/bottom views are rotated by 90°. Buffer A contains 20 mM Hepes, 150 mM NaCl, pH 7.4. Two independent preparations of hCyaAm were used for this experiment.