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. 2017 Feb 10;7:42428. doi: 10.1038/srep42428

Figure 2. Surface Plasmon Resonance analysis of post-vaccination cotton rat serum antibody titers against REG and RMG and correlations with PRNT neutralization titers.

Figure 2

(A,B) The same individual post-vaccination cotton rat sera from Fig. 1 were tested for total antibody binding to the E.coli produced unglycosylated RSV-G protein (REG; Recombinant E. coli produced G) protein (A) or glycosylated RSV-G (RMG; Recombinant Mammalian cell derived G) protein (B) by Surface Plasmon Resonance (SPR). Total antibody binding is represented as SPR resonance units. Statistical significance was tested with one-way ANOVA and Bonferroni multiple comparisons tests. ***p < 0.0001, **p < 0.001, *p < 0.05. (C,D) Relationship between the total anti-G antibody binding to REG (C) or RMG (D) of each individual post-REG vaccination sera (groups C and D) from immunized animals in SPR (shown in Fig. 2A and B for binding to REG and RMG respectively) and post-vaccination PRNT neutralization titers (shown in Fig. 1A) was analyzed with Spearman’s correlations. (E) ELISA binding of post-vaccination cotton rat serum antibodies against RSV B1 virus.