Table 2.

Studies using drugs with estrogenic effects

Author, year [reference]	Animal	N	Intervention	Studied variables	Results
Intervention period: Postnatal and puberty
Cruz et al. 2012 [29]	Sprague–Dawley rats	30	Estradiol valerate Single dose	Sexual hormones Gonadotropin Ovary morphology	↑ E2 ↓ androstenedione = LH ↑ atretic follicles ↓ corpora lutea ↑ cystic follicles
Schulster et al. 1984 [30]	Wistar rats (pubertal)	65	Estradiol valerate Single dose	Sexual hormones Gonadotropin Ovary morphology	↓ E2 ↓ LH ↑ cystic follicles
Brawer et al. 1986 [31]	Wistar rats (pubertal)	50	Estradiol valerate Single dose	Gonadotropin Ovary morphology	↓ LH ↓ FSH ↑ cystic follicles ↑ atretic follicles No CL
Fernández et al. 2010 [32]	Sprague–Dawley rats	30	Bisphenol A 10 days	Sex hormones Ovary morphology	↑E2 ↑ T ↑ cystic follicles ↑ atretic follicles ↓ CL
Marcondes et al. 2015 [27]	Wistar rats	10	Estradiol Benzoate Single dose	Sexual hormones Gonadotropin Ovarian morphology	=T = LH = FSH ↑ cystic follicles No CL
Intervention Period: Adulthood
Hemmings et al. 1983 [35]	Wistar rats	32	Estradiol valerate Single dose	Sexual hormones Gonadotropin Ovary morphology	= T ↓ LH ↑ cystic follicles
Quandt et al. 1993 [34]	Guinea pigs	32	Estradiol-17β 2 days	Sexual hormones Ovary morphology	↑ E2 = androstenedione ↑ cystic follicles ↑ atretic follicles
Zangeneh et al. 2011 [33]	Wistar rats	48	Estradiol valerate Single dose + cold stress simultaneously	Sex hormones Gonadotropin Ovary morphology	↑ E2 = LH = FSH ↑ cystic follicles
McCarthy & Brawer, 1990 [36]	Wistar rats	58	E2 pellets 50 days	Sexual hormones Gonadotropin Ovary morphology	= E2 ↑ cystic follicles No CL

T testosterone, E2 estradiol, LH luteinizing hormone, FSH follicle-stimulating hormone, CL corpus luteum; ↑: increase; ↓ decrease; = equal