Table 1.

Software settings for Hamilton Thorne IVOS 10.2 used in this study

Hamilton Thorne settings
Frames per second	60 Hz
Minimum cell size	10 pixels
Cell intensity	125
Straightness (STR) threshold	80%
Medium VAP cut-off	45 µm/s
Low VSL cut-off	15 µm/s
Low VAP cut-off	25 µm/s
Slow cells motile	No
Static head intensity	0.65–4.90
Static intensity gate	0.50–2.50
Magnification	1.89
Temperature of analysis	37 °C
Type of chamber	Makler