Figure 4. Necrostatin-1s (Nec-1s) prevents angiotensin II induced elastin degradation and aortic inflammation.

Aneurysm-prone aortae were harvested from mice treated with DMSO or 1.6 mg/kg/day Nec-1s 28 days after pump implantation. (A) Aortic sections were stained with Verhoeff’s working elastic stain solution for elastic fibers (black). (B–D) Representative photographs of immunostaining with antibodies specific to CD68 (B), CD206 (C) or CD3 (D). Arrows indicate representative staining of CD3. Scale bars = 100 μm.