Table 1. Detecting and quantifying X4 variants in HIV-1 quasispecies by deep sequencing.
| X4 input % | MiSeq |
Mean | CV % | 454 GS-Junior |
CV % | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| T1 | T2 | T3 | T1 | T2 | T3 | Mean | ||||
| 0.5 | 0.0 | 10.7 | 21.0 | 10.6 | 99 | 0.0 | 0.0 | 0.0 | 0.0 | - |
| 1.0 | 17.6 | 15.0 | 2.9 | 11.8 | 66 | 0.6 | 0.5 | 0.0 | 0.4 | 88 |
| 5.0 | 18.3 | 35.2 | 24.5 | 26 | 33 | 5.0 | 4.2 | 5.3 | 4.8 | 12 |
| 20.0 | 51.9 | 57.4 | — | 54.7 | 7 | 30.0 | 25.2 | — | 27.6 | 12 |
| 50.0 | 61.1 | 78.7 | — | 69.9 | 18 | 56.2 | 53.1 | — | 54.7 | 4 |
| 75.0 | 96.0 | 89.6 | — | 92.8 | 5 | 80.6 | 81.6 | — | 81.1 | 1 |
| 100.0 | 100.0 | 100.0 | — | 100.0 | 0 | 100.0 | 100.0 | — | 100.0 | 0 |
Each CHS11(R5):CHS02 (X4) virus mixture was assessed in triplicate (T1, T2, T3) or duplicate beyond 5%.