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. 2017 Feb 10;7:42166. doi: 10.1038/srep42166

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This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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PA3825^EAL (3 μM final concentration) was added to different concentrations of c-di-GMP (labelled as CdG) and the progress of each reaction measured at specified time points through separation of nucleotides using Resource-Q FPLC, followed by integration of peaks at 254 nm absorbance.