Figure 3. The structure of PA3825^EAL in complex with c-di-GMP/Mg²⁺.

C-di-GMP is shown as yellow sticks and Mg²⁺ ions are represented by magenta spheres. Zoomed views of the β5-α5 loop, highlighted in light-blue, demonstrate the variation in the length of the α5 helix between the apo form and substrate forms of PA3825^EAL. The structural shift in the β5-α5 loop allows Asp160 and Asp 161 to coordinate catalytic metals indicated as M1 and M2.