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. 2017 Feb 10;7:42194. doi: 10.1038/srep42194

Figure 3. JP8 preferentially induces cell death in cancer cells.

Figure 3

(A) JP8 treatment induces cell death in mouse B16-F10 melanoma cells more potently than that in normal mouse AML-12 hepatocyte cells. Cells were grown in 96-well plates and treated with JP8 at 5–100 μM or DMSO (control) for 48 h. Cytotoxicity was measured using cell counting kit-8 (CCK-8) assays. (B) JP8 inhibits cancer cell growth more effectively than its skeleton ECG. B16-F10 cells were grown in 96-well plates and treated with JP8 or ECG at 10–100 μM for 48 h. Cell viability was measured using CCK-8 assays. (C,D and E) JP8 treatment induces apoptosis in B16-F10 cells more potently than that in AML-12 cells. Cells were grown in 12-well plates and treated with JP8 at indicated concentration for 48 h. Apoptosis was detected by flow cytometry using annexin V and PI double staining. Dot plots are representatives and bar graphs are quantified results of three independent experiments expressed as the mean ± SEM, **P < 0.01, ***P < 0.001 compared to the control.