Figure 7. JP8 inhibits melanoma tumor growth in C57BL/6 mice.
Mice were injected with B16 cells (3 × 105) subcutaneously. The next day, mice were randomized and treated with vehicle or JP8 at the indicated doses for 21 days. (A) The tumor volume was calculated based on the width and length of tumors every other day during the whole process. (B) Tumor weight was measured after the mice were sacrificed on day 23. Data are presented as the mean ± SEM (n = 8). *P < 0.05, ***P < 0.001 compared to the control. (C) Photographs of representative tumors from each group are shown. (D) JP8 increases ROS accumulation in tumor tissues. Representative images of DHE-detected ROS in liver and tumor tissues from three independent assays.