Fig. 7.

Histochemical staining of root-associated APase activities in bean hairy roots using ELF-97 phosphate as the substrate. (A) Detection of root-associated APase activities in the root hair zone. (B) Detection of root-associated APase activities in the root tip. Hairy roots were incubated in ELF-97 phosphate solution for 30min. Fluorescence was examined using fluorescence microscopy. Bars = 200 µm. Transgenic hairy roots cultivated on MS medium containing 1.2mM KH₂PO₄ (+P), 0.4mM dNTP (+dNTP), or 0 µM KH₂PO₄ (−P) for 14 d, before being used for staining of root-associated APase activities. CK indicates transgenic bean hairy roots transformed with the empty vector. SgPAP7-OX, SgPAP10-OX, and SgPAP26-OX indicate transgenic hairy roots overexpressing SgPAP7, SgPAP10, and SgPAP26, respectively.