Fig. 1.

The C-terminal fragment of TaMOR-D is essential for transcriptional activation activity. (A) Illustration of the full-length ORF and truncations of TaMOR-D. According to the amino acid position of the conserved domain, the full length (P1) and six truncations (P2–P7) were subcloned into the pGBKT7 vector. (B) Transcriptional activation activity of TaMOR-D in a modified yeast two-hybrid assay. BD represents GAL4-binding domain (BD). P1-BD, P2-BD, P3-BD, P4-BD, P5-BD, P6-BD, and P7-BD indicate the full-length ORF and six truncations (P2–P7) introduced into the BD vector (pGBKT7). BD is the negative control.