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. 2016 Jun 1;67(14):4285–4296. doi: 10.1093/jxb/erw209

Fig. 1.

Fig. 1.

Rice transformation constructs and Southern blot analysis of genomic DNA from transgenic rice lines. A Structure of transgenic constructs 35S and GR. AK and DHPS, coding sequences of mutated lysC and dapA, respectively; 35S pro, CaMV 35S promoter; rbcS TP, coding sequence of the chloroplast-targeting sequence of pea rbcS; Gt1 pro and GluB-1 pro, rice glutelin Gt1 or GluB-1 promoter; LKR, partial sequence of rice LKR/SDH cDNA, with arrows showing the direction of gene transcription; and I-2, second intron of the rice glutelin gene Gt1. B–E Southern blot analysis of genomic DNA using the AK (B–D) or Hyg (E) probe. Total DNA was digested with Eco RI or other restriction enzymes as indicated (B–D). M, marker; P, GR plasmid DNA.