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Expression analysis of AK, DHPS, and LKR/SDH in developing rice seeds. A–E Quantitative RT-PCR analysis of bacterial AK and DHPS and rice AK, DHPS, and LKR transcript levels in developing seeds at 15 DAF in WT and five transgenic lines. F–J Western blot analysis of bacterial AK and DHPS and rice AK, DHPS, and LKR protein extracts from developing seeds at 15 DAF. K SDS-PAGE analysis of total proteins. Equal protein amounts were loaded according to Coomassie brilliant blue stained gels.
