Fig. 4.

Auxin biogenesis is required for the first step of cell fate transition. (A–D) GUS staining of 2-DAC leaf explants from WOX11 _pro :GUS cultured on B5 medium (A and B) and B5 medium with 200 μM yucasin (C and D). (E–H) GUS staining of 4-DAC leaf explants from WOX5 _pro :GUS cultured on B5 medium (E and F) and B5 medium with 200 μM yucasin (G and H). (I, J) qRT-PCR analysis of WOX11 (I) and WOX5 (J) in wild type and yuc1246 mutant leaf explants on B5 medium. Bars show SEM with three biological replicate. Each biological replicate was performed with three technical replicates. *P < 0.05 and **P < 0.01 in two-sample t tests. (K) Overexpression of WOX11 partially reverses the rooting defects caused by 200 μM yucasin treatment. (L) Rooting rate of leaf explants from wild-type Col-0 and 35S _pro :WOX11 on B5 medium with 200 μM yucasin treatment. Bars show SD with three biological replicates. n = 30 in each repeat.

B, D, F, and H are close-up views of the boxed regions in A, C, E, and G, respectively. Scale bars, 500 μm in A, C, E, G; 100 μm in B, D, F, H; and 1mm in K.