Fig. 2.

Silencing in seeds, phytic acid estimation, and seed quality of TaABCC13:RNAi lines. (A) Relative transcript levels of TaABCC13 in different RNAi lines of wheat. Total RNA was isolated at 14 d after anthesis of the primary tiller of non-segregating T₄ RNAi lines, cDNA was prepared from 2 µg of RNA (DNA free) and qRT-PCR analysis was performed using a SYBR Green-based assay. (B) Estimation of total phytic acid in mature wheat grains of transgenic lines. Seeds were collected from the primary tiller of each line. (C) Average seed weight of the TaABCC13:RNAi lines was determined by weighing 50 random seeds. (D) The total protein content of seeds from the TaABCC13:RNAi lines and non-transgenic parent was determined by using the Bradford method. Each bar indicates the mean of three biological replicates (three technical replicates). ** indicates significant differences at P<0.05 (t-test).