Fig. 5.
Emergence of lateral roots in TaABCC13:RNAi lines. (A) Relative transcript level of TaABCC13 in different RNAi lines of T4 wheat seedlings. cDNA was prepared from 2 µg RNA (DNA free) and qRT-PCR analysis was performed using a SYBR Green-based assay. (B) Phenotypic analysis of the roots of C306 and transgenic RNAi lines. Ten seeds from TaABCC13:RNAi and C306 were germinated on half-strength Hoagland media in a hydroponic system and observations were recorded at 10 d after germination. Each bar indicates the mean of three biological replicates (10 technical replicates).