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. 2016 Jun 8;67(14):4403–4413. doi: 10.1093/jxb/erw226

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© The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 2. — Functional analysis of various putative promoters using a maize immature embryo transient expression system. (A) Four GUS constructs that were placed under the regulation of the putative polar or bidirectional promoter of Def1 and Def2, respectively. GUS staining of immature embryos bombarded with (B) the putative promoter of Def2, 2-kb P_zmDef2::GUS; (C) the putative promoter of Def1, 2-kb P_zmDef1::GUS; (D) 635-bp P_zmDef2::GUS; and (E) 635-bp P_zmDef1::GUS. (F) Diagrammatic representation of the construct containing the dual reporter genes GFP/GUS, driven by a 635-bp putative bidirectional promoter. Bombarded immature embryos imaged in (G) bright field mode; (H) dark field model using a GFP filter set; and in (I) bright field mode after histochemical staining. LB, left border; RB, right border; T, terminator. Bars, 0.5mm.