Fig. 5.
Truncation analysis of cis-elements of PZmBD1. (A) Schematic representation of the GFP::mPZmBD1::GUS constructs used for maize immature embryo transient transformation. TSS, transcription start site; T, TATA box; G, G box; W, W box; AC, AC-I/AC-II fused cis-elements; MT, mutated TATA box; RG, region; BD1, the intact bidirectional promoter PZmBD1; bd1-bd23, the series of mutated bidirectional promoters of PZmBD1; ck, maize immature embryos bombarded with empty vector. All mutated bidirectional promoters (mPZmBD1) used GUS and GFP as dual reporter genes in one construct. (B) Quantification for GUS activity of mPZmBD1 in immature embryos. Each bar represents data from between three representative independent bombardments, each bombardment contains nine immature maize embryos transformed with 1 μg plasmid of each constuct.