Fig. 6.
The activity of the different GFP::mPZmBD1::GUS constructs assayed using maize immature embryo transient transformation. (A) to (W) represent the 23 constructs pbd1GG3 to pbd23GG3, respectively. Bright, pictures of the embryos were taken 24h after bombardment under a bright field using a Leica fluorescence stereoscope; GFP, pictures taken under dark field with ultraviolet light and a GFP filter following the bright field pictures; GUS, pictures taken after histochemical staining following the dark field pictures. Each construct was subject to two individual bombardments with six immature embryos; this figure shows one embryo. More information is shown in Supplementary Fig. S6. BD1, full length of the bidirectional promoter, and truncated from (A) 81 to 635, (B) 148 to 635, (C) 208 to 635, (D) 299 to 635, (E) 348 to 635, (F) 383 to 635, (G) 456 to 635, (H) 1 to 163, (I) 1 to 297, (J) 1 to 333, (K) 1 to 383, (L) 1 to 458, (M) 1 to 487, (N) 1 to 506, (O) 1 to 556, (P) 1 to 383 and 572 to 635, (Q) 148 to 635 and a point mutation in the TATA box, (R) 1 to 506 and a point mutation in TATA box 1, (S) 1 to 506 and a point mutation in TATA box 2, (T) 1 to 506 and point mutations in TATA boxes 1 and 2, (U) 1 to 506 and point mutations in TATA box 3, (V) 1 to 506 and point mutations in TATA boxes 1–x3, (W) 333 to 384, and (X) empty vector. Bars, 0.5mm.