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. 2017 Feb 8;5:e2947. doi: 10.7717/peerj.2947

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©2017 Hanapi et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.

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(A) Western blot analysis of the cytoplasmic and nuclear components of Sf9 cells incubated with MrNvc VLPs (25 µg/ml) at different time (0, 4, 8, 12 and 16 h). Both cytoplasmic and nuclear extracts were probed with rabbit anti-MrNv serum (A(i)) and anti-His antibody (A(ii)). (B–C) The orthogonal view from the Z-stack images of the green fluorescence in the nucleus (B) and cytoplasm (C) of Sf9 cells incubated with MrNvc VLPs. Blue fluorescence indicates the cell nucleus. Yellow lines interception shows the location of the green fluorescent spots (F-MrNvc VLPs). Bars, 5 µm.