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Representative demonstration of the fluorescence readout differences between the four arabino/ribo combinations of D1 (donor) and D5 (acceptor). Left: Fluorescence of DNA2a/rD1–DNA3a/rD5; 2.5 μM DNA in 50 mM Na-Pi buffer, 250 mM NaCl, pH 7, λexc = 391 nm. Right: Corresponding image of cuvettes excited by a handheld UV lamp.
