Table 2.
Effect of different variables on cellulase production through CCD
| Run# | Substrate conc. (X1) | Inoculum size (X2) | Yeast extract % (X3) | pH (X4) | Enzyme activity (IU/ml) | Residual value | |
|---|---|---|---|---|---|---|---|
| Observed | Predicted | ||||||
| 1 | 0.5 | 3 | 0.8 | 5.5 | 0.32 | 0.39 | −0.074 |
| 2 | 1.5 | 3 | 0.8 | 6.5 | 1.60 | 1.61 | −0.012 |
| 3 | 1.0 | 4 | 1.0 | 5.0 | 1.10 | 1.19 | −0.09 |
| 4 | 1.5 | 3 | 0.2 | 5.5 | 2.01 | 2.09 | −0.08 |
| 5 | 1.5 | 3 | 0.8 | 5.5 | 1.57 | 1.88 | −0.31 |
| 6 | 2.5 | 3 | 0.8 | 5.5 | 2.57 | 2.83 | −0.26 |
| 7 | 1.0 | 2 | 1.0 | 6.0 | 1.40 | 1.45 | −0.05 |
| 8 | 1.5 | 3 | 1.2 | 5.5 | 1.96 | 2.11 | −0.15 |
| 9 | 1.5 | 1 | 0.8 | 5.5 | 2.10 | 2.30 | −0.20 |
| 10 | 1.0 | 2 | 1.0 | 5.0 | 1.60 | 1.65 | −0.05 |
| 11 | 1.0 | 2 | 0.6 | 5.0 | 1.30 | 1.05 | 0.25 |
| 12 | 2.0 | 4 | 0.6 | 6.0 | 2.70 | 2.41 | 0.28 |
| 13 | 1.0 | 4 | 0.6 | 6.0 | 1.20 | 1.45 | −0.25 |
| 14 | 2.0 | 4 | 1.0 | 6.0 | 1.84 | 1.97 | −0.13 |
| 15 | 2.0 | 2 | 1.0 | 6.0 | 2.50 | 2.36 | 0.13 |
| 16 | 1.5 | 3 | 0.8 | 4.5 | 1.70 | 2.02 | −0.32 |
| 17 | 2.0 | 4 | 0.6 | 5.0 | 2.80 | 2.63 | 0.16 |
| 18 | 1.0 | 4 | 1.0 | 6.0 | 1.20 | 0.93 | 0.26 |
| 19 | 1.0 | 2 | 0.6 | 6.0 | 1.60 | 1.46 | 0.13 |
| 20 | 1.0 | 4 | 1.0 | 5.0 | 1.40 | 1.19 | 0.20 |
| 21 | 2.0 | 4 | 1.0 | 5.0 | 2.89 | 2.80 | 0.09 |
| 22 | 1.5 | 5 | 0.8 | 5.5 | 1.83 | 1.96 | −0.13 |
| 23 | 2.0 | 2 | 0.6 | 5.0 | 2.41 | 2.44 | −0.03 |
| 24 | 1.5 | 3 | 0.8 | 5.5 | 2.10 | 1.88 | 0.21 |
| 25 | 1.0 | 4 | 0.6 | 5.0 | 1.20 | 1.10 | 0.09 |
| 26 | 2.0 | 2 | 1.0 | 5.0 | 3.50 | 3.13 | 0.36 |