Table 1. Steady-State Kinetic Parameters for Wild-Type HEPD and HEPD-E176A.
| system | kcat (s–1) | Km,HEP (μM) | kcat/Km,HEP (M–1 s–1) | Km,O2 (μM) | kcat/Km,O2 (M–1 s–1) | KIE on kcat | KIE on kcat/Km,HEP | KIE on kcat/Km,O2 |
|---|---|---|---|---|---|---|---|---|
| wt HEPD, HEP | 0.30 ± 0.01 | 7.5 ± 1.5 | (4.1 ± 0.8) × 104 | 19 ± 3 | (1.6 ± 0.3) × 104 | 1.0 ± 0.1 | 1.3 ± 0.3 | 5.5 ± 1.3 |
| wt HEPD, D2-HEP | 0.31 ± 0.02 | 10 ± 2 | (3.0 ± 0.5) × 104 | 110 ± 20 | (2.9 ± 0.5) × 103 | |||
| HEPD-E176A, HEP | 0.75 ± 0.06 | 17 ± 2 | (4.5 ± 0.7) × 104 | 7.1 ± 1.4 | (9.9 ± 1.9) × 104 | 1.2 ± 0.1 | 1.5 ± 0.3 | 4.3 ± 1.0 |
| HEPD-E176A, D2-HEP | 0.65 ± 0.02 | 21 ± 3 | (3.1 ± 0.4) × 104 | 28 ± 3 | (2.3 ± 0.3) × 104 | |||