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. Author manuscript; available in PMC: 2018 Jan 3.
Published in final edited form as: Circulation. 2016 Nov 4;135(1):73–88. doi: 10.1161/CIRCULATIONAHA.116.022281

Figure 2.

Figure 2

HFD impairs adrenergic reserve, and induces diastolic and systolic dysfunction in murine hearts. a and b) HFD leads to reduced cAMP and PKA phosphorylation at serine 16 of PLB and at serine 23/24 of troponin I (TnI) in mouse hearts. HFD also leads to reduced serca expression (n=12). *p < 0.05 and **p < 0.01by Student's t-test c) HFD promotes time-dependent systolic and diastolic dysfunction in mouse hearts (n=12). &&p < 0.01, &&&p < 0.001 by repeated measures ANOVA. d and e) After 6 month of HFD, mouse hearts display reduced PKA phosphorylation of PLB (S16, n=6) and contractile response to β adrenergic stimulation (ISO, 0.2 mg/kg in vivo, n=12). ** p < 0.01, and *** p < 0.001 by one-way ANOVA followed by post hoc Tukey's test.