Fig.1.

Abca1 deficiency negatively affects memory of APP transgenic mice. A-D) MWM results from 7-month-old APP mice expressing mouse Abca1 (APP/Abca1^wt; n = 16) or Abca1^ko (APP/Abca1^ko; n = 14). Age- and gender-matched non-APP expressing Abca1^wt (n = 14) or Abca1^ko (n = 16) littermates were used as controls. Analysis is by two-way ANOVA to determine interaction and main effect of genotype or training, and t-test to determine genotype differences in performance within trial days. A, B) Training phase of MWM with each bar representing performance from all trials for a training day. A) Escape latency to the hidden platform: APP/Abca1^wt versus WT, no interaction and no main effect of genotype. APP/Abca1^ko versus Abca1^ko, no interaction and significant main effect of genotype; F(1,140) = 22.26, p < 0.001. B) Path length to the hidden platform: APP/Abca1^wt versus WT, no interaction and no main effect of genotype. APP/Abca1^ko versus Abca1^ko, no interaction and significant main effect of genotype; F(1,140) = 21.09, p < 0.001. For A and B, ^**p < 0.01, ^*p < 0.05 versus non-APP expressing controls by t-test. C and D represents probe trial of MWM performed 24 h following last training trial. Analysis is by one-way ANOVA followed by Tukey’s post-test (shown on the graph). C) Latency to reach the target quadrant of the hidden platform. p < 0.0001. D) Time spent in the target quadrant of the hidden platform. p < 0.05. E) Novel object recognition was performed on a different group of 7-month-old mice of the same genotypes. Shown is percent novel object recognition as indicated in the Methods. Analysis is by one-way ANOVA followed by Tukey’s post-test. n = 8–12 male and female mice per group.