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. 2016 Oct 25;6(12):1267–1272. doi: 10.1002/2211-5463.12143

Figure 1.

Figure 1

Expression of bovine F1 in Escherichia coli. (A–D) Native‐PAGE analysis of water‐soluble fraction of E. coli cells expressing bovine F1 without (plasmid pBF1(‐AFs), lane 2) or with (pBF1(+AFs), lane 3) simultaneous expression of the two genes, ATP11 and ATP12. Lane 1 represents authentic bovine F1 purified from bovine heart muscle. The gels were analyzed by immunoblotting using anti‐β‐subunit (A) or anti‐δ‐subunit antibodies (B), or by protein staining with CBB (C, D). (D) The region containing a band of bovine F1 in C is enlarged. Arrows indicate the band of bovine F1. (E) SDS/PAGE analysis of authentic bovine F1 (lane 1) and purified E. coli‐expressed bovine F1 (lane 2). (F) Inhibition of ATPase activity of bovine F1 by IF1. GFP‐fused IF1 was used. The ATPase activity in the absence of IF1‐GFP is set to 100%. Solid line, E. coli‐expressed bovine F1; dotted line, authentic bovine F1.