Figure 1.

A, Cell line IC₅₀ values assessed for growth inhibition in a 72-hour MTT assay. B, Evidence of PARP cleavage (detected using antibody specific to the cleaved fragment) with ATRi monotherapy for 48 hours at 0.5 µmol/L AZD6738. C, Cell line IC₅₀ sorted by p53 functional status. Differences between groups were compared using the Mann–Whitney test. D, Comparison of dose–response curves to AZD6738 treatment of HCT116 p53 wild-type and HCT116 p53-null cells. Dose–response curves were plotted based on 72 hours MTT. Inset, Western blot represents assessment of p53 function by exposing to radiation.