Figure 2.

Prorenin and (P)RR mRNA and protein expression in endometrial cancer and matched adjacent non-cancerous endometrium. (A) There was no difference in REN mRNA expression between tumour and adjacent non-cancerous endometrium. (B) ATP6AP2 mRNA expression was greater in tumour (*P = 0.023) than that in adjacent endometrium. Scale bars = 500 μm. Images in the bottom panels were taken at a higher magnification, scale bars = 200 μm. Prorenin immunostaining was intense in both glandular (g) and stromal (s) tissue of both the adjacent non-cancerous endometrium (C) and tumour (E and G). ATP6AP2, although intensely expressed in tumour glandular epithelium (F and H), was localised to the perivascular space (closed head arrow) and endothelium (open head arrow) in the adjacent normal endometrium (E). Immunostaining was not seen in the negative controls containing no primary antibody (inserts, tumour tissues).